Indications for Legionnaires' disease testing
For patients with pneumonia, the following indications warrant testing for Legionnaires' disease:
- Outpatient antibiotic failure for community-acquired pneumonia
- Severe pneumonia, in particular illness requiring intensive care
- Presence of an immunocompromising condition
- Overnight travel away from the home during incubation period
- Risk factors and hospitalization with healthcare-associated pneumonia
- Overnight stay in a healthcare facility during incubation period
- Epidemiologic link to a setting with known Legionella concerns, ie, a setting that is currently undergoing an environmental and/or outbreak investigation for legionella.
Testing for Legionella
The Infectious Disease Society of America and the American Thoracic Society (IDSA, ATS) recommend Urine Antigen testing (UAT) for Legionella pneumophila serogroup 1, for persons with
- severe pneumonia that fails to respond to empiric treatment or
- those who have been epidemiologically linked to an outbreak.
However, there are several other tests available and sputum culture remain a goal standard. Medical Facilities should confer with ACDOH to ensure that all Legionella isolates from any clinical specimen are submitted to NYS DOH Wadsworth Center Laboratories
Test Type |
Specimen Type |
Advantages |
Challenges |
Culture (gold standard) |
Lower Respiratory (Sputum)
Tissue
|
Provides confirmatory lab evidence of Legionnaires Disease
Detection of all species and serogroups
WGS can be conducted
Comparison of clinical and environmental isolates to identify potential source.
|
Clinicians must specifically request specimen be cultured for Legionella (not general respiratory bacterial culture) as specialized media (buffered charcoal yeast extract {BCYE}agar) is required.
|
Polymerase Chain Reaction (PCR)
|
Lower Respiratory (Sputum)
Tissue
|
Provides confirmatory lab evidence of Legionnaires Disease.
Detection of L. pneumophila serogroup 1 as well as other species and serogroups.
|
Must be reflexed to culture to perform WGS for comparison to environmental isolates to identify potential source of infection in outbreaks.
|
Urine Antigen |
Urine
|
Provides confirmatory lab evidence of Legionnaires Disease.
Only reliably detects L. pneumophila serogroup 1.
|
Cannot identify or rule out infection of other Legionella species and serogroups.
Cannot be used for WGS.
Cannot be used to identify potential environmental source of infection in outbreaks.
|
Serology |
Blood
|
Can diagnose acute Legionnaires Disease infection retrospectively.
|
A single antibody titer is NOT diagnostic for legionellosis.
Requires collection of second specimen, 3-4 weeks apart, to detect a fourfold rise in antibody titer to a level of >1:128.
Cannot be used to identify a potential environmental source of infection in outbreaks.
|
Reporting Legionella
Providers should report cases to LHD using the DOH-389 Communicable Disease Reporting form found here: health.ny.gov/professionals/diseases/reporting/communicable/. Laboratory specimens should be sent to NYS Wadsworth Center Bacteriology Laboratory
If you are unable to reach ACDOH, contact the NYSDOH BCDC at (518) 473-4439 or by email at epiLegionella@health.ny.gov during business hours or (866) 881-2809 evenings, weekends, and holidays.
Treatment for legionella
Current IDS guidelines for the recommended treatment of Legionella include Fluoroquinolone (levofloxacin or moxifloxacin) or a macrolide (azithromycin preferred) See IDS Guidelines [.pdf]
- Azithromycin 500mg IV/PO q24h
- Levofloxacin 750mg IV/PO q24h
- Moxifloxacin 400mg IV/PO q24h